"In the big moments you need the referee to stay strong, but unfortunately he has bottled it", said Kane.
While England have changed little since their run to the World Cup semifinals, with nine of the starting 11 having played in Russian Federation during the summer, the turnover during Gareth Southgate's two-year reign in charge was evident in this game.
Gareth Southgate gave a start to Marcus Rashford, a player now struggling to make his mark with Manchester United this season.
But referee Danny Makkelie infuriated the hosts by ruling that the forward had fouled David De Gea when collecting a high ball, leading to sharp words from captain Kane.
"There are individual positives and as a collective they gave everything", Southgate said of the trio who were given their chance to make their mark.
"It was a really good team performance and there were some outstanding individual performances and a lot of players who came of age on an worldwide stage".
"We knew it was going to be a hard game, these are the teams we want to play against". Last summer we played friendlies against Brazil, France, Germany and Spain. We represent England and it's great to play around the country. "We want to test ourselves against the best and certainly we put up a fight [against Spain]".
"I think it's clear for everybody to see that it should have been a goal".
"I'm hoping to look after the players and give them a bit of what they need and I know we'll get a good performance because whenever we have changed the team in recent months the level of performance has always remained the same".
The Three Lions also suffered a blow after left-back Luke Shaw was stretchered off with a head injury, but he was later said to be "awake and alert". I think in the end it's not something that's right to discuss publicly. "I am under contract, I have two years, you know that leads to a European Championship, which we have games at Wembley [in] which is going to be a brilliant experience", Southgate told reporters. Now we can go on and improve.